Join us for a webinar on Wednesday, April 11:
Accurate detection of ultra-low frequency variants using xGen® Duplex Seq Adapters
Advances in next generation sequencing (NGS) technology are quickly transforming the field of molecular diagnostics and personalized medicine by allowing analysis of low-input clinical samples. However, low and ultra-low frequency (less than 1%) variants, usually sub-clonal in patient samples, are often confounded by errors introduced by NGS library preparation, target enrichment, and sequencing. Molecular barcoding strategies that label both strands of input DNA have been shown to allow identification and removal of these errors; however, the challenges of producing these specialized adapters and their low library conversion efficiency, hinder their use with limited sample inputs. We have recently developed unique adapters, xGen Duplex Seq Adapters—Tech Access, that are compatible with standard library preparation methods yet permit tagging of double-stranded DNA and statistical reconstruction of duplicate reads. This leads to significantly enhanced error correction, and therefore unprecedented accuracy of low-frequency variant detection, even at a less than 0.5% allele frequency level. Such advances will be key to improving diagnostics and advancing precision medicine.
In this webinar, we will discuss library preparation, target enrichment, and duplex consensus building enabled by xGen Duplex Seq Adapters. Key considerations and recommendations for applications using these adapters will also be presented.